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anti ampk1  (Boster Bio)


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    Structured Review

    Boster Bio anti ampk1
    Anti Ampk1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+ampk1/Anti-Phospho-AMPK+alpha1+(Ser496)PRKAA1+Antibody/pmc12870326-95-4-6
    Average 94 stars, based on 3 article reviews
    anti ampk1 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Sigma-1 Receptor Rescues Autophagy Through AMPK/mTOR Signaling Pathway in Sepsis-Induced Acute Kidney Injury
    Article Snippet: After quantification using the BCA method (Biosharp), 30 μg of protein was separated by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and then transferred onto a polyvinylidene difluoride (PVDF) membrane (Merck Millipore). .. Following a 1-h incubation at room temperature in 5% BSA, the membrane was exposed to the following primary antibodies at 4°C overnight: σ-1R (1:1,000 dilutions; #15168-1-AP; Proteintech), LC3 (1:2,000 dilutions; #14600-1-AP; Proteintech), Beclin 1 (1:1,000 dilutions; #11306-1-AP; Proteintech), AMPK (1:1,000 dilutions; #10929-2-AP; Proteintech), phospho-AMPK1 (S496; p-AMPK1; 1:1,000 dilutions; #BM4718; Boster), mTOR (1:5,000 dilutions; #66888-1-Ig; Proteintech), phospho-mTOR (Ser2448; p-mTOR; 1:5,000 dilutions, #67778-1-Ig; Proteintech), Powered by TCPDF (www.tcpdf.org) ULK1 (1:1,000 dilutions, #20986-1-AP; Proteintech), phospho-ULK1 (Ser556; p-ULK1; 1:2,000 dilutions; #80218- 1-RR; Proteintech), and β-actin (1:5,000 dilutions; #66009-1-lg; Proteintech). .. After washing in Tris-buffered saline with Tween 20 for 10 min three times, the membrane was exposed to horseradish peroxidase (HRP)-labeled Goat anti-rabbit IgG H&L (1:1,000 dilutions, #A0208; Beyotime) or HRP-labeled Goat anti-mouse IgG H&L (1:1,000 dilutions, #A0216; Beyotime) at 37°C for 1 h. Finally, BeyoECL Plus solution (Beyotime) was used to visualize the protein blots with a luminometer.

    Article Title: Sigma-1 Receptor Rescues Autophagy Through AMPK/mTOR Signaling Pathway in Sepsis-Induced Acute Kidney Injury
    Article Snippet: After quantification using the BCA method (Biosharp), 30 μg of protein was separated by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and then transferred onto a polyvinylidene difluoride (PVDF) membrane (Merck Millipore). .. Following a 1-h incubation at room temperature in 5% BSA, the membrane was exposed to the following primary antibodies at 4°C overnight: σ-1R (1:1,000 dilutions; #15168-1-AP; Proteintech), LC3 (1:2,000 dilutions; #14600-1-AP; Proteintech), Beclin 1 (1:1,000 dilutions; #11306-1-AP; Proteintech), AMPK (1:1,000 dilutions; #10929-2-AP; Proteintech), phospho-AMPK1 (S496; p-AMPK1; 1:1,000 dilutions; #BM4718; Boster), mTOR (1:5,000 dilutions; #66888-1-Ig; Proteintech), phospho-mTOR (Ser2448; p-mTOR; 1:5,000 dilutions, #67778-1-Ig; Proteintech), ULK1 (1:1,000 dilutions, #20986-1-AP; Proteintech), phospho-ULK1 (Ser556; p-ULK1; 1:2,000 dilutions; #80218-1-RR; Proteintech), and β-actin (1:5,000 dilutions; #66009-1-lg; Proteintech). .. After washing in Tris-buffered saline with Tween 20 for 10 min three times, the membrane was exposed to horseradish peroxidase (HRP)-labeled Goat anti-rabbit IgG H&L (1:1,000 dilutions, #A0208; Beyotime) or HRP-labeled Goat anti-mouse IgG H&L (1:1,000 dilutions, #A0216; Beyotime) at 37°C for 1 h. Finally, BeyoECL Plus solution (Beyotime) was used to visualize the protein blots with a luminometer.

    Membrane:

    Article Title: Sigma-1 Receptor Rescues Autophagy Through AMPK/mTOR Signaling Pathway in Sepsis-Induced Acute Kidney Injury
    Article Snippet: After quantification using the BCA method (Biosharp), 30 μg of protein was separated by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and then transferred onto a polyvinylidene difluoride (PVDF) membrane (Merck Millipore). .. Following a 1-h incubation at room temperature in 5% BSA, the membrane was exposed to the following primary antibodies at 4°C overnight: σ-1R (1:1,000 dilutions; #15168-1-AP; Proteintech), LC3 (1:2,000 dilutions; #14600-1-AP; Proteintech), Beclin 1 (1:1,000 dilutions; #11306-1-AP; Proteintech), AMPK (1:1,000 dilutions; #10929-2-AP; Proteintech), phospho-AMPK1 (S496; p-AMPK1; 1:1,000 dilutions; #BM4718; Boster), mTOR (1:5,000 dilutions; #66888-1-Ig; Proteintech), phospho-mTOR (Ser2448; p-mTOR; 1:5,000 dilutions, #67778-1-Ig; Proteintech), Powered by TCPDF (www.tcpdf.org) ULK1 (1:1,000 dilutions, #20986-1-AP; Proteintech), phospho-ULK1 (Ser556; p-ULK1; 1:2,000 dilutions; #80218- 1-RR; Proteintech), and β-actin (1:5,000 dilutions; #66009-1-lg; Proteintech). .. After washing in Tris-buffered saline with Tween 20 for 10 min three times, the membrane was exposed to horseradish peroxidase (HRP)-labeled Goat anti-rabbit IgG H&L (1:1,000 dilutions, #A0208; Beyotime) or HRP-labeled Goat anti-mouse IgG H&L (1:1,000 dilutions, #A0216; Beyotime) at 37°C for 1 h. Finally, BeyoECL Plus solution (Beyotime) was used to visualize the protein blots with a luminometer.

    Article Title: Sigma-1 Receptor Rescues Autophagy Through AMPK/mTOR Signaling Pathway in Sepsis-Induced Acute Kidney Injury
    Article Snippet: After quantification using the BCA method (Biosharp), 30 μg of protein was separated by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and then transferred onto a polyvinylidene difluoride (PVDF) membrane (Merck Millipore). .. Following a 1-h incubation at room temperature in 5% BSA, the membrane was exposed to the following primary antibodies at 4°C overnight: σ-1R (1:1,000 dilutions; #15168-1-AP; Proteintech), LC3 (1:2,000 dilutions; #14600-1-AP; Proteintech), Beclin 1 (1:1,000 dilutions; #11306-1-AP; Proteintech), AMPK (1:1,000 dilutions; #10929-2-AP; Proteintech), phospho-AMPK1 (S496; p-AMPK1; 1:1,000 dilutions; #BM4718; Boster), mTOR (1:5,000 dilutions; #66888-1-Ig; Proteintech), phospho-mTOR (Ser2448; p-mTOR; 1:5,000 dilutions, #67778-1-Ig; Proteintech), ULK1 (1:1,000 dilutions, #20986-1-AP; Proteintech), phospho-ULK1 (Ser556; p-ULK1; 1:2,000 dilutions; #80218-1-RR; Proteintech), and β-actin (1:5,000 dilutions; #66009-1-lg; Proteintech). .. After washing in Tris-buffered saline with Tween 20 for 10 min three times, the membrane was exposed to horseradish peroxidase (HRP)-labeled Goat anti-rabbit IgG H&L (1:1,000 dilutions, #A0208; Beyotime) or HRP-labeled Goat anti-mouse IgG H&L (1:1,000 dilutions, #A0216; Beyotime) at 37°C for 1 h. Finally, BeyoECL Plus solution (Beyotime) was used to visualize the protein blots with a luminometer.



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